Journal: PLoS ONE

Article Title: Digital Gene Expression Tag Profiling Analysis of the Gene Expression Patterns Regulating the Early Stage of Mouse Spermatogenesis

doi: 10.1371/journal.pone.0058680

Figure Lengend Snippet: (A) Protein levels of FGF13, SFRP2 and RAC in GC-1spg and GC-2spd (ts) cells were detected by western blotting respectively. β-Actin were used to normalize the individual expression levels. (B) Protein level of WNT10A and FGF7 in the medium supernatants from GC-1spg and GC-2spd (ts) cells were determined by ELISA. The data are presented as the mean plus SEM from a representative of three independent experiments, with each performed in triplicate. Statistically significant differences between GC-1spg and GC-2spd (ts) cells are indicated above the bars: * means P<0.05.

Article Snippet: The antibodies used in Western blotting were RAC (ab13048, Abcam, USA), SFRP2 (12189-1-AP, Proteintech, USA), FGF13 (13201-1-AP, Proteintech,USA) and β-actin (A-4700, Sigma, USA).

Techniques: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques: